GSDMD and infection: Following infection with L. mexicana, neutrophils are rapidly recruited to the site of infection and return to basal levels by 3 days p.i. A second wave of neutrophils arises around 3 weeks post infection [37], a time at which the size of the inflammatory lesion between Gsdmd-/- and C57BL/6 mice does not differ significantly yet (Figs 2F and 7A), and only the amastigote form is present.