Meanwhile, after treatment with RNase A and Triton X-100, the expression of SLC16A1-AS1 in medium of HCC cells decreased significantly (Supplementary Fig. S2B), suggesting that extracellular SLC16A1-AS1 is mainly encapsulated in exosomes with membrane structure instead of being directly released. This evidence concerns the gene SLC16A1 and hepatocellular carcinoma.