Fortunately, in our system, the preferential distribution of ISO-PLGA-DiR and TNFR2-PLGA-DiR in mouse colon cancer in a relatively short time frame is as follows: The nanoparticles started accumulating in the tumor site from 6 h after injection, and the number of accumulated nanoparticles increased over time (Fig. 4A). The gene discussed is TNFRSF1B; the disease is malignant colon neoplasm.