Treatment with ISO-PLGA-ADR nanoparticles resulted in a more profound reduction of Tregs in the tumor (27.9% and 23.1% in CT26 or MC38 tumor-bearing mice; Fig. 7A and B; P < 0.01), while the TNFR2 expression on tumor-infiltrating Tregs remained unchanged (Fig. 7C). Here, TNFRSF1B is linked to neoplasm.