More importantly, in mouse MC38 and CT26 colon cancer models, treatment with TNFR2-PLGA-ADR potently inhibited the growth of tumor, which is associated with a decrease of TNFR2+ Tregs and, consequently, an increase of IFNγ+CD8+ cytotoxic T lymphocytes (CTLs) in the tumor tissue. The gene discussed is TNFRSF1B; the disease is colonic neoplasm.