discovered that administering methotrexate decreased the levels of CD68 macrophages, CD3 T lymphocytes, TNF‐α, caspase‐3, type 1 collagen, TGF‐β, ERK1/2, and SMAD3 protein expression in a mouse model of Marfan syndrome. The gene discussed is TGFB1; the disease is Marfan syndrome.