To validate the results of bioinformatics analysis, the relative NEDD1 expression in LUAD tissues and adjacent non-tumor tissues from eight cases were detected by western blot and analyzed using ImageJ (p = 0.016, Fig. 1D); the results confirmed that NEDD1 was up-regulated in LUAD tissues compared to non-tumor tissues. The gene discussed is NEDD1; the disease is neoplasm.