Closer examination of the gene sets revealed typical (cancer) stem cell markers in cluster 1 (spheres) such as MET, PROM1 (CD133), LGR5, and MYC. The EpCAMhi/lo adherent subpopulations shared gene expression patterns (cluster 5), but differed in the degree of EMT as illustrated by increased ZEB1, SPARC and MMP7 expression in EpCAMlo cluster 2. This evidence concerns the gene MET and cancer.