For example, surface plasmon resonance (SPR), cellular thermal shift assay (CETSA), thermal proteome profiling (TPP) assays revealed that lycorine directly targeted the C-terminal domain of Isocitrate dehydrogenase 1 (IDH1), thereby disrupting its interaction with the deacetylase enzyme sirtuin 1 (SIRT1) and significantly triggering oxidative stress in HCT116 colorectal cancer cells [33]. The gene discussed is SIRT1; the disease is colorectal cancer.