Similar to those findings, steatohepatitis was exacerbated after 18 weeks of feeding mice with a high-fat choline-deficient (HFCD) diet, and RIPK3 and MLKL were sequestrated in the insoluble protein fraction of liver lysates from NASH mice, suggesting early events during steatohepatitis progression [58]. This evidence concerns the gene MLKL and metabolic dysfunction-associated steatohepatitis.