In contrast, increased HSF1 levels characterized tumor stages 1, 2, and 4 (Supplementary Figure 1F; Normal vs. Stage 1, p = 3.03 × 10–09; Normal vs. Stage 2, p = 2.20 × 10–05; Normal vs. Stage 4, p = 7.68 × 10–03). Here, HSF1 is linked to neoplasm.