To have final confirmation of the role of HIF-1α in mediating the enhanced anti-tumor CD8 T-cell function in PHD2/3 KO CD8 T cells, we generated HIF-1α and PHD2/3 double knockout (double KO) OT-1 CD8 T cells and administered them to mice with MC38-OVA tumors. The gene discussed is EGLN1; the disease is neoplasm.