Importantly, despite the accumulation of somatic mutations, the three key amino acids, Trp50HC, Asn58HC, and Arg71HC, that make critical contacts with the CD4-BS of Env (7), remained unaltered, similar to what occurs during the affinity maturation process of VRC01-class bnAbs during HIV-1 infection (4, 5). This evidence concerns the gene CD4 and HIV-1 infection.