Pathway analysis showed that the differential miRNAs were mainly involved in miRNAs in cancer, Human papillomavirus infection, Cellular senescence, Prolactin signaling pathway, signaling pathways regulating pluripotency of stem cells, FoxO signaling pathway, Axon guidance, mTOR signaling pathway, Renal cell carcinoma and Proteoglycans in cancer (Figure 7B). The gene discussed is PRL; the disease is renal cell carcinoma.