The obtained cut-off values of our study cohort were similar to published reference values.9,42 Diagnostic accuracy of pNfH was slightly higher than that of NfL, whereas both Nf strongly correlated with each other, as reported previously.42,43 Since Nf are especially abundant in large myelinated axons, levels are often highest in patients with ALS, as axonal damage contributes to a mass of release into CSF and blood.5 The gene discussed is NEFL; the disease is amyotrophic lateral sclerosis.