Treatment of PBMCs did increase serum perforin and granzyme B. However, the combination of PBMCs with Nb‐TriTE did not lead to further alteration in the serum levels of perforin and granzyme B. Notably, in the tumor region, perforin and granzyme B were significantly increased by Nb‐TriTE combined with PBMCs treatment. Here, GZMB is linked to neoplasm.