NOS3 and Hyperglycemia: The potential contribution of hyperglycemia was analyzed by treating mice with streptozotocin, which induces diabetes associated with endothelial dysfunction and uncoupling of endothelial nitric oxide synthase.31 Streptozotocin induced sustained hyperglycemia in chow-fed mice (Figure 3A) and had no statistically significant effect on TG levels (Figure 3B).