Function prediction and analysis of Actinomyces illustrated that it can induce high expression of TLR2, TLR4, and NF‐κB in young‐onset CRC and reduce infiltration of CD8+T cells in tumor microenvironment, which could provide a direction for the mechanism study of CRC induced by Actinomyces.46 The gene discussed is TLR4; the disease is colorectal carcinoma.