The multivariable logistic regression model analyses identified five statistically significant factors associated with being more likely to develop depression symptoms in adolescent girls with PCOS: perceived social support (OR = 0.944, 95% CI =0.917 ~ 0.971), sleep quality (OR = 1.246, 95% CI =1.131 ~ 1.374), belief illness (OR = 1.082, 95% CI =1.042 ~ 1.123), Hirsutism (OR = 2.530, 95% CI =1.190 ~ 5.377), and LH/FSH ≥ 2 (OR = 2.858, 95% CI =1.400 ~ 5.836) (Table 2). This evidence concerns the gene PLOD1 and depressive symptom measurement.