IGF1 and Follicular Cyst: The serum hormone levels in cows with follicular cysts were also significantly changed, seen by increased FSH (Fig. 1C, 11.73 ± 0.44 IU/L in estrus and 15.00 ± 0.44 IU/L in follicular cyst cows) and LH (Fig. 1D, 359.02 ± 6.99 ng/mL in estrus and 413.97 ± 15.03 ng/mL in follicular cyst cows), together with significantly decreased E2 (Fig. 1E, 96.64 ± 4.14 pmol/L in estrus and 77.60 ± 2.93 pmol/L in follicular cyst cows), E2/P4 (Fig. 1G, 3.22 ± 0.21 in estrus and 2.55 ± 0.10 in follicular cysts) and IGF (Fig. 1H, 55.75 ± 2.67 ng/mL in estrus and 43.17 ± 0.64 ng/mL in follicular cyst cows).