Treatment with Cas9mIL30-mPSCA NxPs suppressed IL30 expression and resulted in multiple foci of necrobiosis and ischemia associated with impaired microvessel density (MVD) and reduced cancer cell proliferation (ANOVA: p < 0.0001) compared with those in tumors treated with empty-mPSCA NxPs or PBS (Fig. 8m and Supplementary Table 13). This evidence concerns the gene IL27 and cancer.