To further explore the development of CD8+ T cells in NPC, we first performed pseudotime trajectory analysis and revealed four developmental paths from CD8_C1_LEF1, CD8_C7_ZNF683, CD8_C8_CXCL13, and CD8_C10_STMN1 cells at initial or mediate states to CD8_C11_HAVCR2 at terminal state (Fig. 7c and Supplementary Fig. 13c). This evidence concerns the gene LEF1 and nasopharyngeal carcinoma.