Based on the aforementioned conclusion, given that CD14+ myeloid cells can differentiate into Mono/Macro upon interaction with various cell types such as alveolar, cancer, and endothelial cells, and considering that macrophages are among the most abundant immune subsets capable of presenting MHC and activating T cells upon stimulation24, we proceeded to evaluate the distribution patterns of CD11c (monocyte marker) and CD68 (macrophage marker) using multiplex immunofluorescence. Here, CD14 is linked to cancer.