Regarding immune cell composition, primary and metastatic tumors were mainly characterized by CD8+ Texh, CD4+ Treg, neutrophils, and CD4+ conventional T cells (Tconv), whereas the para-tumor region was dominated by granulocyte lineages, including neutrophils, monocytes, and macrophages (Supplementary Fig. S1a). This evidence concerns the gene CD4 and metastatic neoplasm.