To directly address the role of Lyve1-expressing macrophages in the development of atherosclerosis, we generated Lyve1cre+/WTCsf1rflox/flox mice (and Csf1rflox/flox controls) on an apolipoprotein-E-deficient (Apoe−/−) background to selectively ablate LYVE1+ macrophages21 and assessed the effect of their genetic deletion on atherosclerosis. This evidence concerns the gene APOE and atherosclerosis.