To investigate whether the AhR-ILC3-IL22 pathway was necessary in I3C-mediated prevention of microbial dysbiosis caused by colitis, 16S rRNA bacterial profiling was performed on fecal samples from female WT C56BL/6 and AhRΔRorc experimental mice induced with or without colitis and/or treated with I3C. This evidence concerns the gene AHR and colitis.