In HCC tissues, the m5C modification level of mRNA is significantly greater than that in normal tissue, and growth factor receptor-bound protein 2 (GRB2), ring finger protein 115 (RNF115) and apoptosis antagonizing transcription factor (AATF) are the top NSUN2-related m5C hypermethylated genes (78). This evidence concerns the gene RNF115 and hepatocellular carcinoma.