We found that coculture of THP-1 cells with HIV-1LAI-infected SUPT1 cells (Figure 1B) but not mock-infected SUPT1 cells results in robust inflammasome activation as indicated by IL-1β secretion, suggesting that our coculture system, which lacks DEAE-dextran, can induce HIV-dependent inflammasome activation via cell-to-cell infection (Figure 1C). The gene discussed is IL1B; the disease is infection.