We found that inflammasome activation following cell-to-cell transmission of HIV-1 could be detected by 24 hours (Figure 3B), which is delayed relative to our detection of CARD8 inflammasome activation 2 hours post cell-free HIV-1 infection in the presence of DEAE-dextran (Kulsuptrakul et al., 2023). The gene discussed is CARD8; the disease is HIV-1 infection.