NUP153 and Alzheimer disease: Dot blot assay revealed that Aβ accumulated in AD organoids compared with control ones while its level was decreased in Nup-AD organoids (Fig. 5B optical density: C-GFP 1 ± 0.05, AD-GFP 1.97 ± 0.06, AD-Nup153-GFP 0.83 ± 0.25 P < 0.05 ANOVA followed by Bonferroni test, n = 3–4).