We found that the mean fluorescence intensity (MFI) for DHE was higher in AD-NSCs compared with WT-NSCs, but it was reduced in AD-NSCs overexpressing Nup153 (DHE MFI: WT-NSC-GFP 22,173 ± 3,096; AD-NSC-GFP 45,351 ± 3,316; AD-NSC-GFP-Nup153 19,713 ± 2,047, ANOVA with Bonferroni test P < 0.001, n = 4; Fig. 2B). Here, NUP153 is linked to Alzheimer disease.