The activation of NLRP3 involves the cleavage of pro-IL-18 and IL-1β, as well as the release of high-mobility group box 1 (HMGB1), which requires lysosomal rupture, calcium influx, and ROS production from mitochondria, making it closely linked to the development of AP (Hoque et al. 2011, 2014). This evidence concerns the gene HMGB1 and alkaline phosphatase measurement.