Necroptosis, triggered by factors released in the early phase of AP in response to protease activation, involves the formation of the necrosome involving receptor-interacting serine/threonine-protein kinase 1 (RIPK1) or RIPK3, resulting in the phosphorylation of mixed lineage kinase domain-like (MLKL), ultimately resulting in membrane rupture (He et al. 2016). This evidence concerns the gene MLKL and alkaline phosphatase measurement.