Immunohistochemical assays of Ki67 (antigens associated with proliferating cells, more colored dots represent high expression of cell proliferation) and TUNEL (apoptotic cells fluorescing in green) revealed a reduction in the number of proliferative tumor cells and an increase in the number of apoptotic cells, validating the excellent therapeutic outcome of the +L+D group. This evidence concerns the gene MKI67 and neoplasm.