NUCB2 and Insulin resistance: We found that the body weight, blood glucose, insulin resistance and erectile function of the treated mice were significantly decreased, and the expression of contractile phenotypic marker α-SMA was increased, while the synthetic phenotypic marker OPN was decreased, the corpus cavernosum smooth muscle fibers were increased, and PI3K/AKT/mTOR signaling pathway was activated.We established an in vitro model of spongy smooth muscle cultured with high oleic acid and high glucose and treated it with Nesfatin 1.