TRPC6 and focal segmental glomerulosclerosis: In contrast with the healthy physiological state and similarly to another preclinical FSGS model using Pod-GCaMP3 mice in our earlier study (18), in no-drug control, Pod-GCaMP5/tdTomato TRPC6-Tg mice had visual signs of ongoing FSGS pathology, including segmental elevations in podocyte calcium, the development of multiple parietal podocytes and adhesions between parietal Bowman’s capsule and glomerular capillary segments, and albumin leakage through the GFB (Figure 4, A–J).