To corroborate these findings in vivo, we implanted the murine melanoma cell line B16-F10 (B16) tagged with fluorescent protein YFP orthotopically in mice and compared LDH expression and glucose uptake potential between B16-YFP tumor cells and CD8+ TILs by flow cytometry after 2 weeks of tumor progression (Supplemental Figure 1). Here, CD8A is linked to melanoma.