As shown in Figures 2o and p, the protein levels of pyroptosis markers, including NLRP3, caspase-1 p20 and GSDMD-N, were suppressed by BRD3308, which indicated that BRD3308 may alleviate sepsis-induced ALI by inhibiting pyroptosis in macrophages. This evidence concerns the gene NLRP3 and Sepsis.