DUOX2 and alkaline phosphatase measurement: In order to ensure the reliability and accuracy of the results, we will carry out in vivo experiments to build and verify the mouse model of acute pancreatitis, and then use gene editing technology to build miR-605-3p or DUOX2 knockout or knock in mouse models, and induce AP on these models, observe the pathogenesis, inflammation and tissue damage of AP, verify that miR-605-3p/DUOX2 axis is involved in the progression of AP, and our results may provide a promising therapeutic strategy for AP in the future.