To evaluate the impact of NLRP3 deletion on early organ infiltration, tissue-specific survival in a non-tumor-primed environment, and proliferation during leukemic cell seeding into bone marrow and spleen, we conducted a competitive experiment by injecting equal amounts of carboxyfluorescein succinimidyl ester (CFSE)-labeled MOLM-13 ctrl cells and eFluor 450 (eFluor450)-labeled ΔNLRP3 cells (or vice versa) into immunodeficient NSG-S mice. Here, NLRP3 is linked to neoplasm.