NPM1 and leukemia: As expected, the most frequently mutated genes were DNMT3A (19.32%) and NPM1 (20.29%), followed by FLT3 (13.53%) which is consistent with the AML mutation landscape [14] as shown in Additional file 1, Table S1-d, reinforcing the relevance of our DA1-3b leukemia model for future translational applications in humans.