Mycobacterial HSP65 was detected only in the phagosomal fraction, suggesting that M. bovis BCG resides primarily in the phagosomes during the first 24 h after infection, while IFNγ was found predominantly in the cytosolic fraction but also in the phagosomal fraction where mycobacteria were present (Fig. 3A), suggesting a possible interaction of mycobacteria with IFNγ inside phagosomes. Here, IFNG is linked to infection.