Additionally, results also presented that the percentage of CD68 + /CD163+ macrophages was increased after cocultured with 3‐MA treated AIM2‐overexpressed HCC cells (Figure 5B, C), Meanwhile, the inhibitory effect of AIM2 overexpression on TGF‐β level was partially offset by 3‐MA in coculture system (Figure 5D). This evidence concerns the gene CD163 and hepatocellular carcinoma.