The LOD of FI‐based assays for detecting cancer cell‐derived EVs in F‐PBS using FITC‐labelled CD63‐BP aptamer was determined as 2.0 × 108 HT‐29 EVs/mL (Table 2), the LOD using FITC‐labelled HER2‐HApt aptamer was 5.0 × 108 SKBR3 EVs/mL (Table 2). This evidence concerns the gene CD63 and cancer.