Depletion studies of monocarboxylate transporters (MCT)1‐4 and sodium‐coupled MCT (SMCT)1‐2 showed that only the depletion of MCT1, which is highly expressed in NSCLC tissues, led to reduced levels of acetate, acetyl‐CoA, and synthesized fatty acids in tumor cells or mouse lung tumors. The gene discussed is SLC16A1; the disease is non-small cell lung carcinoma.