To simplify the method we have demonstrated that a double layer of the cationic polymer polyethyleneimine (bridging layer) and the anionic polymer hyaluronan (external layer) is stable enough to attain coated NPs entrapping a combination of chemotherapeutics in both the core and the shell with specificity toward CD44 overexpressing cancer cells (Maiolino, Moret, et al., 2015; Maiolino, Russo, et al., 2015; Russo et al., 2016). Here, CD44 is linked to cancer.