RB1 and neoplasm: Our data also illustrated that several of the most altered regions in NB showed correlations with aggressive tumour behavior that included older age at diagnosis, higher stage tumour and amplification of MYCN. The strongest correlation was observed for NNAT/BLACP and RB1 and MAGEL2 and the tumours that underwent methylation alterations at these regions were > 1.5 years of age, had stage 4 tumour and were MYCN amplified.