Here, we have investigated the spatiotemporal dynamics of lysosomes, using live-cell imaging of lysosomal associated membrane protein 1 (LAMP1)-GFP-transfected non-malignant prostate epithelial cell lines (PNT1a, PNT2 and PWR1E) and prostate cancer cell lines (22Rv1, LNCaP, DU145, and PC-3; Fig. 1a and Supplementary Videos 1). Here, LAMP1 is linked to prostate cancer.