Using the Cancer Genome Atlas Prostate Adenocarcinoma (TCGA-PRAD [20]) dataset, we found significant alterations in lysosomal trafficking genes in prostate cancer patients that were consistent across Gleason patterns 3, 4, and 5 (GP3, GP4, and GP5), indicating a sustained disruption in lysosomal trafficking machinery during disease progression (Fig. 2a and Supplementary Tables 2, 5). Here, GP5 is linked to Familial prostate cancer.