Targeting helicases (DDX21, DDX56, RAD54B), amino acid transporters (SLC1A5, SLC38A2, SLC38A1), and nuclear pore complex/transport components (TPR, NUP155, XPO1) decreased cancer cell line viability most significantly compared to that of normal cells, while we also observed a tendency to decrease viability across the tested cell lines in the case of blocking DNA repair/processing activity (RUVBL1, RUVBL2) and targeting chaperones (HSPA9, HSPA1B, HSPA2). This evidence concerns the gene RUVBL2 and cancer.