In this conventional in vitro model of AP, statistically significant increases in necrosis induced by 10 nM CCK (p = 0.043) and 100 nM caerulein (p = 0.040) were observed in the cells isolated from WDD and LNCA-deficient ATG16L1[E230] mice (Figure 4B). This evidence concerns the gene CCK and alkaline phosphatase measurement.