When we inhibited the ISR pathway during ZIKV infection using ISRIB, a broad ISR inhibitor (Fig. 3), or GSK2606414, a PERK inhibitor (data not shown), ATF4 protein expression was reduced, and ATF3 mRNA levels were negligible (Fig. 3, and data not shown). Here, EIF2AK3 is linked to Zika virus infectious disease.