We asked if the specific TARDBP mutations contribute to BMEC dysfunction, however, TARDBPG295S/G295S and TARDBPK263E/K263E gene-edited hiPSCs-derived EECM-BMEC-like cells didn’t show impaired diffusion barrier functions nor enhanced immune cell adhesion compared to the isogenic control same as the ALS patient (TARDBPN345K/+)-derived clone. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.