In fact, we found that ALS patient (TARDBPN345K/+)-derived EECM-BMEC-like cells showed significantly decreased expressions of AXIN2, CLDN5, and TTYH2, which were reported as the canonical Wnt-target genes (Gastfriend et al., 2021b; Arribas et al., 2024) (Figure 7A). Here, TTYH2 is linked to amyotrophic lateral sclerosis.