Furthermore, flow cytometry analysis of NRCMs with Annexin V-APC/7AAD staining revealed thatEEPD1 shRNA infection significantly exacerbated apoptosis after radiotherapy, with no discernible difference in apoptosis between theEEPD1 shRNA and control shRNA groups in the absence of radiotherapy (Figure 4C). This evidence concerns the gene ANXA5 and infection.