This study also showed that down-regulation of Notch signaling by glycyrrhizin resulted in increased up-regulation of pro-apoptotic proteins such as Bad and Bax and down-regulated expression of anti-apoptotic proteins such as Bcl-2.135 Treatment of CaSki cervical cancer cells with glycyrrhizin has been shown to suppress proliferation, lead to mitochondrial dysfunction and decrease Notch1 mRNA expression.136 High mobility group box 1 (HMGB1), which triggers EMT, was targeted by glycyrrhizin in prostate cancer and glycyrrhizin was shown to inhibit HMGB1.137. The gene discussed is HMGB1; the disease is prostate carcinoma.