We also showed that Gr-1+ cells composed of Gr-1+CD11b+F4/80− PMNs and Gr-1+CD11b+F4/80+ monocytes/macrophages in Spl in tumor-bearing WT mice and tumor-bearing CD11c:DTA mice had a similar inhibitory effect on the division of anti-CD3/CD28-stimulated eFluorTM 670-labeled CD4+ T cells (Supplementary Figures 15A, B in the Supplementary Material), indicating that they act as MDSCs (39). Here, CD4 is linked to neoplasm.