TNF and Alzheimer disease: We analyzed the mRNA levels of TNF-α, IFN-γ, IL-1β, IL-4, IL-6, IL-13, TSLP, and RANTES in DNCB-induced AD-like skin lesions using RT-qPCR (Figures 3A–H), and the protein levels of TNF-α, IgE, histamine, IL-4, and IL-13 in DNCB-induced mouse serum using ELISA (Figures 3I–M).