The mRNA levels of TNF-α, IL-1β, IL-4, IL-6, IL-13, TSLP, and RANTES were increased in the AD cell model constructed by TNF-α/IFN-γ-induced HaCaT cells, and protein levels of IL-1β, IL-4, IL-6, and IL-13 were also elevated. This evidence concerns the gene TNF and Alzheimer disease.